Tetrazolium-stained embryo axisMany tropical seeds are ‘recalcitrant’ and lose viability quickly. This poses a major challenge in the seed conservation in the tropics. For recalcitrant seeds, cryopreservation is a promising strategy for long-term seed preservation but successful procedures for long-term preservation are yet to be established for many species. Using germination tests and tetrazolium vital staining, I assessed the viability of seeds and excised embryonic axes (units often used for cryopreservation) after storing them in liquid nitrogen. We tested various desiccation and freezing treatments and showed that tolerance to desiccation and freezing differed among tissues within the embryonic axis (tissues stained in red retained bioactivity). Treatments that led to retention of bioactive meristem may be promising for subsequent tissue cultures (Sugiyama et al. 2016).